Here are the steps:
1. Create the first ROI (plus symbol in Track filtering) – you should see a sphere in the surface view and circles in the volume view.
2. Go to the volume view, press ctrl (command for mac) and move the circl to desired location.
3. Add another ROI – you should see sphere of different color. Select it by clicking on the ROI description in the track filtering menu. This means you have selected the desired ROI.
4. Go to volume view and move it around. You should see tracks that pass through both spheres.
Consider utilizing Python libraries like DIPY or tools like MATLAB to accomplish real-time fiber display between two ROIs. Utilize interactive visualization tools such as Mayavi or PyVista, define your ROIs, and extract pertinent tractography data. Use GPU acceleration or multithreading to maximize performance for more fluid real-time rendering.
To achieve real-time fiber display between two ROIs, use tractography software like DSI Studio or MRtrix. Define the ROIs, run the fiber tracking algorithm, and visualize the connectivity dynamically. Ensure optimal parameter settings for accuracy.