When adding new Regions of Interest (ROIs) to BrainSuite software, it’s essential to ensure that the new ROIs are accurately defined and appropriately integrated into the existing framework. Begin by clearly identifying the anatomical or functional regions you wish to include, and use high-resolution imaging to delineate these areas precisely. After defining the ROIs, validate them by comparing with existing data or expert annotations to ensure consistency and reliability. This process enhances the analytical capabilities of BrainSuite, facilitating more detailed and accurate brain studies.